BS EN ISO 12966-3-2009 动物和植物的脂肪和油.脂肪酸甲基酯的气相色谱分析.使用三甲基氢氧化硫制备甲基酯

标准号：BS EN ISO 12966-3-2009
中文标准名称：动物和植物的脂肪和油.脂肪酸甲基酯的气相色谱分析.使用三甲基氢氧化硫制备甲基酯
英文标准名称：Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Preparation of methyl esters using trimethylsulfonium hydroxide
标准类型：X14
发布日期：2010/1/31 12:00:00
实施日期：2010/1/31 12:00:00
中国标准分类号：X14
国际标准分类号：67.200.10
引用标准：ISO 661
适用范围：This part of ISO 12966 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils including milk fat and blends containing milk fat. Isomerization of unsaturatedfatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit.As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA). As CLA is not correctly analysed, this method is not applicable to the determination of the complete fatty acid composition of milk fat samples.Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.NOTE This part of ISO 12966 is based upon German Standard Method C-VI 11e (98) (see Reference [8]).