标准号:ISO 8128-2-1993
中文标准名称:苹果汁、浓缩苹果汁和含苹果汁的饮料 棒曲霉素含量的测定 第2部分:薄层色谱法
英文标准名称:Apple juice, apple juice concentrates and drinks containing apple juice; determination of patulin content; part 2: method using thin layer chromatography
标准类型:X50
发布日期:1999/12/31 12:00:00
实施日期:1999/12/31 12:00:00
中国标准分类号:X50
国际标准分类号:67.160.20
适用范围:The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 鎔/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.