BS EN 14486-2005 水质.单分子层噬菌斑序列检测人体肠道病毒

标准号：BS EN 14486-2005
中文标准名称：水质.单分子层噬菌斑序列检测人体肠道病毒
英文标准名称：Water quality - Detection of human enteroviruses by monolayer plaque assay
标准类型：Z16
发布日期：2005/6/27 12:00:00
实施日期：2005/6/27 12:00:00
中国标准分类号：Z16
国际标准分类号：07.100.20
适用范围：This European Standard describes a method for the detection of those serotypes of enterovirus that replicate and form plaques in BGM cells under agar. These will largely be poliovirus and Coxsackievirus B serotypes. Most serotypes of echovirus, Coxsackievirus A and animal enteroviruses are unlikely to produce plaques under these conditions. Reoviruses are unlikely to be detectable within the specified seven day incubation period. It is applicable to any type of water and processed water sample although toxic elements may interfere with cell culture. The methods to confirm that the plaques seen contain enterovirus are given. Methods to identify which serotype of enterovirus is found in the plaque are not included as this is not required by the EU Bathing Water Directive. Basic cell culture procedures including the preparation, maintenance and enumeration of BGM cell culture are not described but suggestions are made for media that may be used (see Annex A).