DIN EN 12014-3-2005 食品.硝酸盐和/或亚硝酸盐含量测定.第3部分:硝酸盐酶催化还原成亚硝酸盐后肉制品中硝酸盐和/或亚硝酸盐含量的光谱测定

标准号：DIN EN 12014-3-2005
中文标准名称：食品.硝酸盐和/或亚硝酸盐含量测定.第3部分:硝酸盐酶催化还原成亚硝酸盐后肉制品中硝酸盐和/或亚硝酸盐含量的光谱测定
英文标准名称：Foodstuffs - Determination of nitrate and/or nitrite content - Part 3: Spectrometric determination of nitrate and nitrite content of meat products after enzymatic reduction of nitrate to nitrite; German version EN 12014-3:2005
标准类型：X04
发布日期：1999/12/31 12:00:00
实施日期：1999/12/31 12:00:00
中国标准分类号：X04
国际标准分类号：67.120.10
适用范围：This European Standard specifies a spectrometric method for the determination of nitrate and nitrite content of meat products and has been validated for different meat products with a content of 9 mg/kg to 22 mg/kg nitrite calculated as sodium nitrite and 23 mg/kg to 48 mg/kg nitrate calculated as sodium nitrate. Experiences have shown that the method is also applicable for total nitrite and nitrate content from 5 mg/kg up to 125 mg/kg calculated as sodium nitrite. Nitrite in an aqueous extract of the test sample is treated with sulfanilamide and N-(1-naphthyl)- ethylenediamine dihydrochloride. The formed red compound is measured spectrometrically at a wavelength of 540 nm. Nitrate in an aqueous extract of the analytical sample is converted into nitrite by nitrate reductase. This converted nitrite together with the nitrite which is already in the analytical sample reacts with sulfanilamide and N-(1-naphthyl)ethylenendiamine dihydrocloride. The colour intensity of this resulting red compound is measured in a spectrometer at 540 nm. The nitrate content is calculated from the difference between the spectrometric measurements.