BS EN 12821-2009 食品.高效液相色谱法测定维生素D含量.维生素D3或钙化醇(维生素D2)的测定

标准号：BS EN 12821-2009
中文标准名称：食品.高效液相色谱法测定维生素D含量.维生素D3或钙化醇(维生素D2)的测定
英文标准名称：Foodstuffs - Determination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D3) or ergocalciferol (D2)
标准类型：X04
发布日期：2009/4/30 12:00:00
实施日期：2009/4/30 12:00:00
中国标准分类号：X04
国际标准分类号：67.050
引用标准：EN ISO 3696
适用范围：This European Standard specifies a method for the determination of vitamin D3 (cholecalciferol) or vitamin D2(ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC).Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Bothvitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable forsamples with a content of vitamin D3 and vitamin D2.Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the correspondingmetabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. ThisEuropean Standard does only include measurement of vitamin D3 or vitamin D2.This European Standard provides the base for the analytical methods. It is intended to serve as a frame inwhich the analyst can define his own analytical work in accordance to the standard procedure.This method has been validated in inter-laboratory tests on fortified and non-fortified samples such asmargarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4μg/100 g to 14 μg/100 g. Further information on the validation data is given in Annex D.