DIN ISO 16000-17-2010 室内空气.第17部分:霉菌的测定与计算.培养基方法(ISO 16000-17-2008)

标准号：DIN ISO 16000-17-2010
中文标准名称：室内空气.第17部分:霉菌的测定与计算.培养基方法(ISO 16000-17-2008)
英文标准名称：Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008)
标准类型：C51
发布日期：2010/6/1 12:00:00
实施日期：1999/12/31 12:00:00
中国标准分类号：C51
国际标准分类号：13.040.20
引用标准：ISO 8199;ISO 16000-16;ISO 16000-18
适用范围：Mould spores are widely distributed in the outdoor environment and, therefore, occur in varying concentrations also indoors. Growth of moulds in indoor environments, however, should be considered a hygienic problem because epidemiological studies have revealed that dampness and/or mould growth in homes and health problems affecting the occupants are closely related. Harmonized methods for sampling, detection and enumeration of moulds including guidelines for sampling strategies are important for comparative assessment of mould problems indoors. Sampling is carried out according to the method described in DIN ISO 16000-16 or -18. Agar plates (DG 18 agar and malt-extract agar or potato extrose agar) obtained from sampling by impaction are incubated directly at (25 +/- 3) ?C and (36 +/- 2) ?C. Filters obtained from sampling by filtration are re-suspended in saline solution (0, 9 % NaCl) with 0, 01 % Polysorbate 80. Decimal dilutions of the suspension are prepared and aliquots spread on DG 18 agar as well as on malt-extract agar or potato dextrose agar (indirect method). Agar plates are incubated at (25 +/- 3) ?C. For special purposes plates can be incubated at (36 +/- 2) ?C (e. g. thermotolerant Aspergillus spp.) or (45 +/- 2) ?C (Aspergillus fumigatus). After incubation, mould colonies are identified and counted.